Magerus-Chatinet, Aude Frédéric Rieux-Laucat
Published in
Methods in Molecular Biology
The apoptosis function can be monitored on human lymphocytes by quantifying the induced-death upon apoptotic stimuli involving either the extrinsic or the intrinsic pathway on in vitro activated T cells. Her, we describe an in vitro assay allowing the monitoring of three different apoptosis pathways: (1) the FAS-induced pathway, (2) the activation-...
Julie Chaumeil Augui, Sandrine Chow, Jennifer C Heard, Edith
Published in
Methods in Molecular Biology
Epigenetic mechanisms lead to the stable regulation of gene expression without alteration of DNA and trigger initiation and/or maintenance of cell-type-specific transcriptional profiles. Indeed, modulation of chromatin structure and the global 3D organization of the genome and nuclear architecture participate in the precise control of transcription...
Oudina, Karim Cambon-Binder, Adeline Delphine Logeart-Avramoglou
Published in
Methods in Molecular Biology
Bioluminescence from murine stem cells tagged with the luciferase gene reporter and distributed within three-dimensional scaffolds of two different materials is quantified in vitro and in vivo. The luminescence emitted from cells adhering to the scaffolds tested is monitored noninvasively using a bioluminescence imaging system. Monitoring the kinet...
Lozach, Pierre-Yves Burleigh, Laura Staropoli, Isabelle Ali Amara
Published in
Methods in Molecular Biology
DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures present on viral glycoproteins and function as attachment factors for several enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (cis-infection). They are also able to capture viruses and tran...
Loredana Saveanu Peter Van Endert
Published in
Methods in Molecular Biology
Cross-presentation is defined as the ability of certain professional antigen-presenting cells to take up, process and present extracellular antigens on major histocompatibility class I (MHC-I) molecules to CD8+ T cells. The stimulation of naive cytotoxic CD8+ T cells by this process, termed cross-priming, is involved in many different responses, in...
Diana, Julien Beaudoin, Lucie Gautron, Anne-Sophie Agnès Lehuen
Published in
Methods in Molecular Biology
NKT cells are innate-like αβ T cells that are conserved between humans and mice. They are distinct from conventional T cells as they recognize lipid antigens presented by the CD1d molecule. Most NKT cells expressed a highly restricted TCR repertoire and can be activated by α-galactosylceramide (α-GalCer) and detected by α-GalCer-loaded-CD1d tetrame...